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Gas chromatography-mass spectrometric (GC-MS) procedure is presented for the simultaneous qualification and quantitation of methamphetamine (MA), amphetamine (AMP), 3,4-methyl enedioxymethamphetamine (MDEA), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxyethylamphetamine (MDEA) in human hair, The method procedure involves decontamination of hair with distilled water and acetone, acidic hydrolysis, extraction in the presence of deuterated internal standards, and GC-MS analysis after derivati3ation with trifluoroacetic anhydride (TFAA) in ethylacetate, The limit of detection for 5 drugs were about 0.1~0.15 ng/mg using 30 mg hair sample. Coefficient variations of correlation ranged from 0.9941 to 0.9993. The recoveries of these drugs were found to be 93.4~104.4%. The concentrations of AMP, MA, MDA, and MDMA in abusers’ hair samples were measured 0.17~2.88, 2.09~18,34, 0.24~3.83, and 3.10~22.81 ng/mg, respectively. The ratios of MA/AMP and MDMA/MDA ranged 5.67~49.57 and 4.78~54.31, respectively, This assay has been successfully utilized in the evaluation of the deposition of amphetamine-type stimulants (ATS) in human hair.
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